Gaga was moments away from performing Shallow - the hit song from A Star is Born, in which she starred with Cooper - before she had words with the crowd.
“And one more thing, be kind or f**k off,” she said.
Gaga and Cooper made headlines for their ‘insane’ chemistry in the hit flick.
The 33-year-old recently addressed the rumours during an appearance on Jimmy Kimmel.
“First of all, social media, quite frankly, is the toilet of the internet and what it’s done to pop culture is abysmal.”
“People saw love and, guess what, that’s what we wanted to you to see. This is a love song. The movie, A Star Is Born, it’s a love story.”